《植物生理学报》 2015, 51(11): 2025-2030

兴仁金线兰种子非共生萌发特性与快繁育苗

周丽^1,*, 隆林², 王苑¹, 王兴益¹, 刘森银³

¹兴义民族师范学院, 民族药用生物资源研究与开发重点实验室, 贵州兴义562400; ²黔西南州药品检验所, 贵州兴义562400; ³黔西南州绿缘动植物科技开发有限公司, 贵州兴义562400

通信作者：周丽；E-mail: zhouli@xynun.edu.cn

摘　要：

本文研究了兴仁金线兰种子非共生萌发特性和芽增殖条件, 结果表明, 授粉后55 d 种子达到生理成熟状态, 种子萌发率最高达97.28%; 授粉后75 d种子趋于形态成熟, 萌发率可达94.44%; 授粉后115 d的种子过度成熟, 萌发率反而仅为23.34%。芽增殖的最佳培养基为ER+0.3 mg•L^-1 6-BA+0.5 mg•L^-1 NAA+0.03 g•L^-1活性炭, 增殖系数为8.25。把试管苗移栽到直径6~9 mm的细树皮中, 成活率在96.83%以上。本研究为兴仁金线兰的产业化生产奠定了基础。

关键词：兴仁金线兰; 非共生萌发; 快繁育苗

收稿：2015-09-15   修定：2015-10-14

资助：贵州省教育厅产学研结合示范基地(黔教合KY字[2012]035号)、贵州省教育厅2011年教育质量提升项目(黔教高发[2011]278号)、贵州省教育厅创新团队(黔教合人才团队字[2013]30号)。

Asymbiotic Seed Germination and Rapid Seedling Regeneration of Anoectochilus xingrenensis

ZHOU Li^1,*, LONG Lin², WANG Yuan¹, WANG Xing-Yi¹, LIU Sen-Yin³

¹Key Laboratory of National Medicinal and Biological Resources, Xingyi Normal University for Nationalities, Xingyi, Guizhou 562400, China; ²Qianxinan Prefecture Institute for Drug Control, Xingyi, Guizhou 562400, China; ³Southwest Guizhou Lvyuan Animal and Plant Technologies Company Limited, Xingyi, Guizhou 562400, China

Corresponding author: ZHOU Li; E-mail: zhouli@xynun.edu.cn

Abstract:

This paper introduces a high quality plantlets propagation technology of Anoectochilus xingrenensis developed by researching its characteristics of the asymbiotic seed germination and suitable condition of bud multiplication. Results showed that the seeds of 55 days after pollination (DAP) reached physiological maturity, with a seed germination rate up to 97.28%; the seeds of 75 DAP could be morphologically mature, with a seed germination rate up to 94.44%; while the seeds of 115 DAP were over-mature, with a seed germination rate dropping to 23.34%. Eriksson medium with 0.3 mg•L^-1 6-BA, 0.5 mg•L^-1 NAA and 0.03 g•L^-1 active charcoal (AC) was optimal for bud multiplication, with an effective multiplication coefficient of 8.25. The survival rate could be up to 96.83% when the plantlets were transplanted to 6–9 mm diameter pine bark. Therefore, this research provides a basis for the industrial production of A. xingrenensis.

Key words: Anoectochilus xingrenensis; asymbiotic germination; rapid seedling regeneration